Contrast staining with methylene-green-pyronine solution. The most common stain for blood smears. It is mainly used for identifying plasma cells, whose cytoplasm is stained red because of the high content of RNA, and to detect RNA and DNA in tissue sections.
The preparation is fixated for 3 minutes in May-Grünwald stain, diluted with an equal quantity of Aqua destillata and stained for 3 minutes. The solution is poured off and the preparation is further stained with Giemsa solution for 15-20 minutes.
- P. G. Unna, in:
Monatshefte für praktische Dermatologie, 1902, 55: 76.
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