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Henri-Michaelis-Menten equation

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An equation for enzyme-catalysed biological reactions relating the rate of formation of product to the concentration of enzyme and of substrate (reactant). This equation gives an expression for the rate of an enzyme reaction and became fundamental to the interpretation of how an enzyme reacts on its substrate. Before the work of Michaelis and Menten, Victor Henri in 1903 found that enzyme reactions were initiated by a bond between the enzyme and the substrate. From 1910 Michaelis and Menten took this work further, investigating the kinetics of an enzyme, saccharase, that catalyzes the hydrolysis of sucrose into glucose and fructose.

We thank Andre Trombeta for information submitted.


  • V. Henri:
    Lois Générales de l’Action des Diastases. Paris, Hermann, 1903.
  • L. Michaelis and M. L. Menten:
    Die Kinetik der Invertinwerkung. Biochemische Zeitschrift, 1913, 49: 333.
    The kinetics of invertase activity.

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