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Donath-Landsteiner test

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A serological test for paroxysmal haemoglobinuria. The antibody is directed toward the P-antigen complex which is located on the surface of red blood cells. Blood from the patient is cooled to 4-5 °C, and a cold haemolysin in the plasma combines with the red blood cells if the patient has the illness. Upon rewarming to 37 °C, the sensitized red cells are haemolysed by the complement normally present.


  • J. Donath, K. Landsteiner:
    Über paroxysmale Hämoglobinurie.
    Münchener medizinische Wochenschrift, 1904, 51: 1590-1593.
    Über Kältehämoglobinurie. Ergebnisse der Hygiene, Bakteriologie, Immunitätsforschung und experimentellen Therapie, Stuttgart, 1925, 7: 184-228.
We thank Rudolf Kleinert, Bad Reichenhall, Germany, for information submitted.

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