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Köhler's principle
Also known as:
Köhler illumination
Associated persons:
Alban Köhler
Description:
A procedure for adjusting a microscope's condenser height and apertures in a way that optimises object illumination. Köhler's principle is based on the concept that the best resolution occurs when all elements of the microscope are in perfect alignment and the iris diaphragms are properly adjusted to the best aperture. The principle therefore calls for focusing the field iris in the same plane as the specimen.
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