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Mallory's phloxine stain

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A technique based on retention of phloxine by hyaline after overstaining and then decolorizing with lithium carbonate, used in combination with alum haematoxylin to give nuclear staining.

Description

A technique based on retention of phloxine by hyaline after overstaining and then decolorizing with lithium carbonate, used in combination with alum haematoxylin to give nuclear staining; hyaline appears red, older hyaline is pink to colourless, amyloid is pale pink, and nuclei are blue-black.

Submitted by Zoran Bojanic, M.D., Serbia.

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