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Sahli's method I

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One of the original techniques for measuring haemoglobin calorimetrically, with a haemometer. The Sahli haemometer method utilizes the conversion of haemoglobin into acid haematin which has a brown colour in solution. The intensity of the colour is related to the amount of haemoglobin in the blood sample. Water is added to dilute the brown solution until it matches that of a standard. The more haemoglobin, the more water required to obtain a colour match. Haemoglobin values are read at the meniscus of the brown solution.

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